PROJECT: SAV-FM-EHP-2008-02-01

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Biosafe transgenic oilseed rape through innovative biotechnology

One of the priorities of EU is the production of nutritionally valuable crops that are more resistant to various types of environmental stress. Genetically modified plants represent a potential tool to achieve this goal undertl_files/images/UGBR SAV 1B.JPG condition of solved questions concerning their bio-safety. Especially the presence of genes for resistance to antibiotics in the genome of transgenic plants represents a risk due to their potential to spread into environment. On the other hand, these genes play inevitable role during processes of regeneration of transgenic plant from transformed tissue. After obtaining transgenic plants, these selectable marker genes become superfluous. In recent few years, a strategy of transgene removal from genome of transgenic plants has been suggested, that exploits Cre/loxP self excision system.

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The proposed project is focused on implementation of this excision system on oilseed rape that has increasing importance both food, as well as bio-fuel. In our system the excision of undesired gene is trigged by the strong, specific cruciferin (cruC) promoter that is solely active in developing seeds of transgenic plants. The result of this process is expected to be a transgenic oilseed rape with eliminated risk of undesired gene flow into environment.


One of the aims of the project is also dissemination of scientific results and evoking discussions on GMOs with both scientific and laic community. We have presented our results in scientific journal, at conferences as well as regular reports for the ministry. Moreover, safety issues with respect to consumers and environment are being discussed in the pleasant environment of coffeeteries (link to the fig) or meetings with students.