Other EU Funded Projects

 

Plant adaptation to heavy metal and radioactive pollution
(7RP, p. no. 612587, 2014 - 2017)
Partners: Belarusian State University (Belarus), Institute of Experimental Botany, The Academy of Sciences of the Czech Republic (Czech Republic), Georg-August-Universität Göttingen (Germany), Aberystwyth University (Great Britain), Federal State Autonomous Educational Institution of Higher Education Siberian Federal University, All Russian Research Institute for Silviculture and Mechanization of Forestry (Russian Federation), M.G.Kholodny Institute of Botany National Academy of Sciences of Ukraine, Institute of Cell Biology and Genetic Engineering National Academy of Sciences of Ukrain (Ukraine)
Anthropogenic activities continue to impact the environment causing soil and ground water contamination in many regions and the effects are often persistent especially where heavy metals and radioactive substances have been released. Ecological problems can be particularly severe in the case of atomic power facilities and recent occurrences (Japan) indicate that the Chernobyl incident in 1986 was by no means unique. Moreover, such accidents hold a rofound "dread" factor for the general population. Environmental pollution can be equally profound and even more persistent in the case of mining waste which contaminates large areas of Europe with diverse heavy metals. Plants adapt to chronic radiation and heavy metal contamination as demonstrated by re-colonization of polluted areas. This project aims to understand the basic principles of protective mechanisms and how such pollution affects the stability of the genome. We propose to establish a research network to evaluate and exploit unique resources in the Chernobyl zone and in mining sites (Wales, UK) as "open area" laboratories for studying how changes of DNA are coordinated with internal cellular networks during plant response to these pollutants. We use a combination of genetic, cell biological, molecular and evolutionary strategies. Part of the project is devoted to crop plants and their ability to grow in contaminated sites with the idea to increase productivity and safety. For the first time, eight research teams with complementary experience in radiation and other plant stresses will cooperate for solving the common for Europe problem of survival in contaminated nature. This will provide the insights on an increasingly detailed knowledge of the regulatory mechanisms for plant stress tolerance and gives an opportunity to see how the problems of Chernobyl and other contaminated places could be solved by scientists and what could be done in order to secure human life against environmental pollution.
Project leader:
Mgr. Martin Hajduch, PhD.

Systematic MSE-based quantification of allergenic and celiac disease proteins in wheat grain
(Contracted by Syngenta, 2010-2012)
Recent developments in the field of mass spectrometry have established MS-based quantification as a reliable technique for identifying specific proteins and their concentrations in complex matrices. When compared with classical quantitative proteomics approaches based on two-dimensional protein electrophoresis (2-DE), MS-based quantification provides three primary benefits. First, many proteins that cannot be detected on 2-DE gels, such as low abundance proteins, can be quantified by MS-based approach. Second, for those proteins that can be identified by their constituent peptides, the identity of the full protein can be verified as part of each sample analysis. Third, a true quantitative measure of protein concentration, along with a multiplex approach and high sample throughput, eclipse the electrophoretic techniques. Among various MS-based techniques, MSE allows one to quantify proteins in complex mixtures based on the concentration of a standard protein that is spiked into analyzed sample. This technique uses an integrated approach comprising parallel, alternating scans of each sample at 1) low-collision energy to obtain precursor ion information, and 2) high-collision energy, to obtain full-scan accurate mass data in a single run (1). The MS spectra obtained at different collision energies are stored separatly. During data acquisition the quadrupole analyzer is not mass selective but is operated in the radio-frequency only mode. Thus, all ions pass to the TOF analyzer. This yields exact mass measured fragment ions that will be potentially observed for every peptide precursor ion present in the low-energy TOF dataset. Therefore, based on the known concentration of each standard protein in the sample it is possible to calculate concentrations of all pre-selected and identified proteins.
Project leader:
Mgr. Martin Hajduch, PhD.

Biosafe transgenic oilseed rape through innovative biotechnology
(SAV-FM-EHP-2008-02-01, 2009-2011)
Project leader: Ing. Jana Libantová, CSc.


The quantitative proteomics analysis of developing embryo, endosperm and seed coat in control and Chernobyl-grown plants

(MIRG-CT-2007-200165, 2007-2011)
Project leader:
Mgr. Martin Hajduch, PhD.

The role of the cytoskeleton and vesicular trafficking in polarity establishment during in vitro embryogenesis and pollen development in Arabidopsis and maize
(AvH 3.4 - 8151/08028, 2008-2009)
Project leader: Mgr. Bohuš Obert, PhD.

MAR sequences as a booster of flax genetic transformation
(ESF, JPD 3BA 2005/1-031, 2007-2008)
Project leader:
Ing. Andrea Hricová, PhD.


The polarity establishment and studies on the cytoskeleton structure and changes in maize androgenesis
(SAS-HAS, 2007-2009)
Project leader: doc. RNDr. Anna Preťová, DrSc.

Programmed cell death in plant reproduction
(SAS - CSIC, 2007-2009)
Project leader:
doc. RNDr. Anna Preťová, DrSc.


The study of mitogen-activated protein kinase (MAPK) signaling to the actin cytoskeleton

(DAAD 62 223, 2005-2006)
Project leader:
doc. RNDr. Ján Salaj, DrSc.